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Laboratory Safety Training Suite

Laboratory safety depends on every employee's participation and cooperation. This suite describes common hazards associated with laboratory environments, including hazardous waste and potentially infectious materials. You will learn how to avoid exposure to hazards and what to do in the event of spills and emergencies. This suite is ideal for anyone who works in a laboratory setting.

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Course Outline

Lab Safety

1. Introduction
1. Welcome
2. Introduction
3. Learning Objectives
2. Chemical Hygiene Plan
1. The Chemical Hygiene Plan
3. Routes of Exposure
1. Routes of Exposure
2. Knowledge Check: Routes of Exposure
4. Chemical Hazards and Effects
1. Chemical Hazards
2. Effects of Exposure
3. Signs and Symptoms of Exposure
4. Exposure Limits
5. Knowledge Check: Exposure Limits
5. Chemical Information
1. Chemical Information
2. Methods of Detection
3. Control Methods
6. Engineering Controls
1. Engineering Controls
2. The Purpose of Lab Hoods
3. Working with Lab Hoods
4. Knowledge Check: Incident Prevention
7. Administrative Controls and PPE
1. Administrative Controls
2. Personal Protective Equipment
3. Attire
4. Eye Protection
5. Gloves
6. Respiratory Protection
7. Personal Hygiene
8. Other Lab Hazards
1. Other Common Laboratory Hazards
2. Handling Glassware

9. Cylinders
1. Cylinder Transport
2. Cylinder Storage
3. Knowledge Check: Proper Storage Practices
10. Electrical Hazards
1. Electrical Hazards
2. Extension Cords
11. Ergonomics
1. Laboratory Ergonomics
2. Good Ergonomic Practices
12. General Safety Rules
1. Other General Rules of Lab Safety
2. Unattended Operations
13. Emergency Response
1. Emergency Response
2. In Case of Fire
3. Safety Showers
4. Eyewash Stations
5. Knowledge Check: Emergency Response
14. Spills, Housekeeping and Disposal
1. Spills
2. Housekeeping
3. Waste Disposal
15. Conclusion
1. Summary

Biosafety Hazardous Waste Handling and Disposal (US)

1. Introduction
1. Welcome
2. Biotechnology and Hazardous Waste
3. Knowledge Check: Biotechnology and Hazardous Waste
4. Lesson Focus
5. What You’ll Learn
2. Hazardous Waste Definition & Your Role
1. Hazardous Waste
2. Hazardous Waste Regulations
3. Your Role
4. Learning Activity – Hazardous Waste Identification
5. Hazardous Waste Management
6. Learning Activity – Hazardous Waste Management Hierarchy
3. Waste Minimization
1. Waste Minimization: Strategies
2. Waste Minimization: Everyday Items
3. Knowledge Check: Minimization 1
4. Knowledge Check: Minimization 2
4. Segregation
1. Segregation
2. Segregation Areas
3. Segregation: Labels
4. Knowledge Check: Segregation 1
5. Knowledge Check: Segregation 2
6. Segregation: Containers 1
7. Segregation: Containers 2
8. Segregation: Containers 3
9. Wastes with Multiple Hazards
10. Learning Activity – What’s Wrong Here?

5. Reusing, Recycling and Disposal
1. Reuse
2. Recycling, Treatment and Land Disposal
3. Where Does Waste Go?
4. Knowledge Check: Disposal 1
5. Knowledge Check: Disposal 2
6. More Basic Concepts
1. Shipping
2. Unknown Wastes
3. What Else You Need to Know
4. Knowledge Check: Unknown Waste
5. Knowledge Check: Basic Concepts
7. Conclusion
1. Conclusion

Understanding Risk (US)

1. Introduction
1. Welcome
2. Introduction
3. Learning Objectives
2. Routes of Exposure
1. Routes of Exposure
2. Ways Exposure Can Occur at Work
3. Cuts and Punctures
4. Ingestion and Absorption
5. Inhalation
6. Activity: Routes of Exposure
3. Classification Systems
1. Classification Systems: Risk Groups
2. Biological Agents by Risk Group
3. Resources for Classifying Agents
4. Activity: Risk Groups
5. Classification of Biosafety Levels
6. Considerations
7. Risk Groups and Biosafety Levels
8. Biosafety Level Designations
9. Activity: Biosafety Level Designations

4. Risk Assessment
1. Assessing Agents
2. Reservoir and Portal of Escape
3. Method of Transmission and Routes of Entry
4. Susceptible Hosts and Incubation Period
5. Activity: Assessing Agents
6. Risk Management
7. Managing Risks
8. Break the Chain of Infection
9. Ways to Break the Chain of Infection
10. Activity: Risk Assessment
5. Conclusion
1. Summary

Controlling Risk (US)

1. Introduction
1. Welcome
2. Introduction
3. Learning Objectives
2. Engineering Controls
1. Facility Design
2. Design Considerations
3. Waste
4. Biological Safety Cabinets (BSCs)
5. BSCs: Environmental Protection
6. Activity: BSCs
7. Activity: Design Considerations
3. Administrative Controls
1. Labels
2. Signs
3. Activity: Administrative Controls 1
4. Work Practice Controls
1. General Precautions
2. Work Areas
3. Hand and Shoes
4. Laboratory Cleanliness Definitions
5. Disinfection
6. Pipetting
7. Biological Material
8. Centrifuging
9. Activity: Work Practice Controls

5. BSCs, Sharps and Waste
1. Proper Use of Biological Safety Cabinets (BSCs)
2. BSCs: Airflow
3. BSCs: Ventilation, Healing and Materials
4. BSCs: Recommended Practices
5. Needles and Other Sharps
6. Glassware
7. Contaminated Sharps
8. Activity: Broken Glass
9. Activity: Contaminated Sharps
6. Personal Protective Equipment (PPE)
1. Gloves
2. Glove Material, Replacement and Removal
3. Lab Coats and Uniforms
4. Eye and Face Protection
5. Surgical Masks and Respirators
6. Activity: Lab Coats and Uniforms
7. Activity: PPE
7. Spill Procedures and Emergencies
1. Spill Control Plan
2. Spill Response
3. Spill Locations
4. Spill Procedures
5. Emergencies
6. What Do I Do If I Am Exposed?
7. Activity: Emergencies
8. Conclusion
1. Conclusion